Double-Stranded Polydeoxyribonucleotides Containing 6-Thiodeoxyguanosine and 6-Thiodeoxyinosine. Poly[d(A-C) . d(T-s6G)], Poly[d(A-s6G) . d(T-C)] and Poly[d(A-C) . d(T-s6I)]
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Ligation of double-stranded and single-stranded [oligo(dT)] DNA by vaccinia virus DNA ligase.
Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed to either a poly(dA) or a poly(rA) backbone and, remarkably, free oligo(dT). Th...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1972
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1972.tb01848.x